In all samples, rates of mitochondrial respiration CO 2 efflux, gray lines Figures 4E—G were similar. The implication of this conclusion is that DNP-INT fails to block the Mehler reaction during in vitro experiments, or the carbon reduction cycle in intact samples.
However, the O 2 uptake rate did not increase commensurately, potentially due to unknown complexities of ROS scavenging within thylakoid membranes. Through these experiments, we demonstrated that measurements of Net O 2 fluxes with an O 2 electrode are insufficient for measurements of the site-specific O 2 reduction pathways operating within thylakoid membranes, due to the complexities of the competing routes of superoxide formation and quenching that potentially invalidate some assumptions underpinning the interpretation of Net O 2 fluxes.
Nonetheless, the chemical may still find use for its apparent ability to slow electron transport between the PQ pool and PSI. This could be useful as a tool to artificially replicate the induction of photosynthetic control, imparted by the Cyt -b6f complex by an acidified lumen, potentially in the absence of NPQ or state transitions. Finally, the disagreement in results between characterization studies of DNP-INT in purified complexes and our findings from intact samples may point to currently unknown aspects of Cyt -b6f function.
Or perhaps the results reflect a sub-population of DNP-INT sensitive Cyt -b6f which may correlate to some spatial distribution in the thylakoid membrane, or its participation in super or mega complexes. Further work to explain the apparent discrepancies of DNP-INT function between spectroscopic measurements in highly purified samples and our results obtained with isolated thylakoids and intact leaf discs may yield significant information about photosynthesis in the future.
The datasets generated for this study are available on request to the corresponding author. DF and AT carried out the experimental work, the data analysis and interpretation of the data. DF drafted the manuscript. AT and E-MA revised the content for final submission.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. We thank Dr. Achim Trebst. Barbagallo, R. Effects of inhibitors on the activity of the cytochrome b 6 f complex: evidence for the existence of two binding pockets in the lumenal site.
Biochemistry 38, — Beckmann, K. On-line mass spectrometry: membrane inlet sampling. Photosynthesis Res. Borisova-Mubarakshina, M. Oxidation of the plastoquinone pool in chloroplast thylakoid membranes by superoxide anion radicals. FEBS Lett. Busch, F. Photosynthetic gas exchange in land plants at the leaf level. Methods Mol. C3 plants enhance rates of photosynthesis by reassimilating photorespired and respired CO2. Plant Cell Environ.
Chain, R. On the interaction of 2,5-dibromomethylisopropylbenzoquinone DBMIB with bound electron carriers in spinach chloroplasts. Fan, D. Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants.
Photosynth Res. Furbank, R. Oxygen exchange associated with electron transport and photophosphorylation in spinach thylakoids. Acta , — Heyno, E. Plastid alternative oxidase PTOX promotes oxidative stress when overexpressed in tobacco. Khorobrykh, S. Oxygen reduction in a plastoquinone pool of isolated pea thylakoids. PubMed Abstract Google Scholar. Krieger-Liszkay, A. Inhibition of electron transport at the cytochrome b 6 f complex protects photosystem II from photoinhibition.
Lam, E. Characterization of electron transfer from water to plastocyanin catalyzed by resolved electron transfer complexes from chloroplasts. Malkin, R. Malnoe, A. Photosynthetic growth despite a broken Q-cycle. Mubarakshina, M. The production and scavenging of reactive oxygen species in the plastoquinone pool of chloroplast thylakoid membranes. Mus, F. Inhibitor studies on non-photochemical plastoquinone reduction and H 2 photoproduction in Chlamydomonas reinhardtii.
Porra, R. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy.
Roberts, A. Biochemistry 40, — Stepien, P. Contrasting responses of photosynthesis to salt stress in the glycophyte Arabidopsis and the halophyte thellungiella: role of the plastid terminal oxidase as an alternative electron sink.
Plant Physiol. Tiwari, A. Photodamage of iron-sulphur clusters in photosystem I induces non-photochemical energy dissipation. Plants Trebst, A. Inhibitors in the functional dissection of the photosynthetic electron transport system. The inhibition of photosynthetic electron flow in chloroplasts by the dinitrophenylether of bromo- or iodo-nitrothymol. The compound is moderately acidic, with a pK A of 4. At pH 2. DNP can also be used as a reagent to detect potassium and ammonium ions.
Unable to display preview. Download preview PDF. Skip to main content. This service is more advanced with JavaScript available. Advertisement Hide. Biological activities of 2,4-dinitrophenol in plant-soil systems. Authors Authors and affiliations P. Shea J. Weber M. Conference paper. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access. Abdel-Rahman, M. Bailey: Responses of sweet and field corn to treatments with dinoseb. Weed Sci. Google Scholar. Alexander, M. Lustigman: Effect of chemical structure on microbial degradation of substituted benzenes. Food Chem. CrossRef Google Scholar. Arnon, D. Allen, and F. Whatley: Photosynthesis by isolated chloroplasts. General concepts and comparison of three photochemical reactions. Acta 20 , Bandal, S.
Casida: Metabolism and photoalteration of 2- sec -butyl-4, 6-dinitrophenol DNBP herbicide and its isopropyl carbonate derivative dinobuton acaricide.
Barber, D. Koontz: Uptake of dinitrophenol and its effect on transpiration and calcium accumulation in barley seedlings. Plant Physiol. Beevers, H. Berlin, J. Barz, H. Harms, and K. Harder: Degradation of phenolic compounds in plant cell cultures. FEBS Letters 16 , Bonner, J. Millerd: Oxidative phosphorylation by plant mitochondria. Bottrill, D. Hanson: The action of 2,4-dinitrophenol on corn root mitochondria.
Cheng, C. Anderson: Flocculation of clays and soils by organic compounds. Soil Sci. Christie, A. Leopold: Entry and exit of indoleacetic acid in corn coleoptiles. Plant and Cell Physiol. Coffmann, L. Woodbridge: Effects of gamma radiation on aqueous solutions of phenol. Couderchet, J. Comptes Rend. Cowan, C. White: Adsorption by organo-clay complexes.
Ninth Annual Conf. Clays Clay Mineral. Davies, J. New York: Elsevier Davis, D. Funderburk, Jr. Weeds 6 , Selman: Effects of water vapor upon the movement of dinitroweed killers in soils. Weeds 3 , 11 Bolt: Determination of anion adsorption by clays. Douros, J. Reed: Decomposition of nitro aromatic compounds. Bacteriol Proc. Dowler, C. Baughman, and C. Ellis, B. Towers: Degradation of aromatic compounds by sterile plant tissues. Evans, W. Smith: The photochemical inactivation and microbial metabolism of chlorophenoxyacetic acid herbicides.
Fogg, G. Applied Biol. Forward, F. Cheung: The metabolism of glucose in corn coleoptiles in relation to oxygen and 2,4-dinitrophenol. Gaur, B. Beevers: Respiratory and associated responses of carrot discs to substituted phenols.
Germanier, R. Wuhrman: Uber den aeroben mikrobiellen Abbau aromatischer Nitroverbindungen. Pathologia et Microbiologia 26 , Gibson, D. Science , Glass, A. Bohm: The uptake of simple phenols by barley roots. Green, R. Guenzi ed. Madison, WI: Soil Sci. Gunderson, K. Jensen: A soil bacterium decomposing organic nitro compounds. Acta Agr. Harel, S. Reingold: The effect of 2,4-dinitrophenol on translocation in phloem. Harris, C. Warren: Adsorption and desorption of herbicides by soil.
Weeds 12 , Hartford, W. Nicholas ed. II: Preservatives and preservative systems, pp. New York: Syracuse Univ. Press Huang, P. Wang, M. Wu, and N. Hsu: Retention of phenolic acids by noncrystalline hydroxy aluminum and iron compounds and clay minerals of soils. Humphreys, T. Jensen, H. Kandler, O. Klepper, L. Krul, W. Jackson, and B. Sidwell: Structure and activity relationships of 2,4-dinitrophenols and other metabolic perturbers on initiation of roots on mung bean cuttings. Leithe, W.
Loomis, W. Lipmann: Reversible inhibition of the coupling between phosphorylation and oxidation. PubMed Google Scholar. Madhosingh, C. Massini, P. Voorn: Effect of ferredoxin and ferrous ion on chloroplastsensitized photoreduction of dinitrophenol. McCormick, N. Feeherry, and H. Levinson: Microbial transformation of 2,trinitrotoluene and other nitroaromatic compounds.
Mellor, R. Salisbury: Influence of light regime on the toxicity and physiological activity of herbicides. Miller, R.
Sopper and L.
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